{"id":3227,"date":"2022-11-02T11:58:52","date_gmt":"2022-11-02T02:58:52","guid":{"rendered":"https:\/\/www.infront.kyoto-u.ac.jp\/?p=3227"},"modified":"2022-11-02T11:58:52","modified_gmt":"2022-11-02T02:58:52","slug":"post-3221","status":"publish","type":"post","link":"https:\/\/www.infront.kyoto-u.ac.jp\/en\/post-3221\/","title":{"rendered":"Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a"},"content":{"rendered":"<p>Riki Ishibashi1,2*, Ritsuko Maki1, Satsuki Kitano3, Hitoshi Miyachi3 and Fumiko Toyoshima1,2*<br \/>\n1Department of Biosystems Science, Institute for Life and Medical Sciences, Kyoto University<br \/>\n2Department of Mammalian Regulatory Networks, Graduate School of Biostudies, Kyoto University<br \/>\n3Reproductive Engineering Team, Institute for Life and Medical Sciences, Kyoto University<\/p>\n<p><strong>Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a<\/strong><\/p>\n<p>Scientific Reports (2022) https:\/\/doi.org\/10.1038\/s41598-022-22639-6<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Riki Ishibashi1,2*, Ritsuko Maki1, Satsuki Kitano3, Hitoshi Miyachi3 and Fumiko Toyoshima1,2* 1Department of Biosystems Science, Institute for Life and Medical Sciences, Kyoto University 2Department of Mammalian Regulatory Networks, Graduate School of Biostudies, Kyoto University 3Reproductive Engineering Team, Institute for Life and Medical Sciences, Kyoto University Development of an in vivo cleavable donor plasmid for targeted [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":3223,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_locale":"en_US","_original_post":"https:\/\/www.infront.kyoto-u.ac.jp\/?p=3221","footnotes":""},"categories":[1],"tags":[],"labo":[],"labo_en":[72],"class_list":["post-3227","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-achievements","labo_en-labo32","en-US"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/posts\/3227","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/comments?post=3227"}],"version-history":[{"count":3,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/posts\/3227\/revisions"}],"predecessor-version":[{"id":3230,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/posts\/3227\/revisions\/3230"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/media\/3223"}],"wp:attachment":[{"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/media?parent=3227"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/categories?post=3227"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/tags?post=3227"},{"taxonomy":"labo","embeddable":true,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/labo?post=3227"},{"taxonomy":"labo_en","embeddable":true,"href":"https:\/\/www.infront.kyoto-u.ac.jp\/wp-json\/wp\/v2\/labo_en?post=3227"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}