Nano Life Science Institute (WPI-NanoLSI),
Kanazawa University

Vu Quang Cong

Real-time quantification of branched-chain amino acids (BCAAs) such as valine, leucine, and isoleucine provides a powerful approach for interrogating amino acid metabolism and its contribution to cellular function. Fluorescence lifetime-based biosensors, combined with fluorescence lifetime imaging microscopy (FLIM), enable robust and quantitative measurements that are independent of biosensor concentration and imaging conditions. Here, we aim to develop a genetically encoded fluorescence lifetime-based BCAA biosensor for live-cell quantification. Using this tool, we will investigate how BCAA metabolism is dynamically regulated and how its rewiring contributes to cancer progression.