KTH Royal Institute of Technology,
Department of Gene Technology (SciLifeLab)

Ian Hoffecker

This collaboration will develop proximity-seq, a sequencing-based 3D imaging platform which integrates droplet microgel encapsulation with DNA barcode network reconstruction. We will focus on organoids and spheroids as model systems, using molecular sequencing rather than optics to map biomolecules in space. The project combines microfluidic expertise at Kyoto University with molecular programming and graph-based spatial inference approaches developed at KTH, enabling high-throughput, automated analysis of tissue microenvironments at single-cell resolution.