Institute for Frontier Life and Medical Sciences, Kyoto University

No. 1308 (Lecture 1) Post-transcriptional Regulation of the E. coli General Stress Response / (Lecture 2) A novel ribosome rescue mechanism in Bacillus subtilis

Date: November 8, (Fri) 2019 ( Lecture 1)13:30~14:50 (Lecture2)15:00~15:45
Room: Seminar Room 104 , 1st floor, Bldg. #2 of Institute for Frontier Life and Medical Sciences ,Kyoto University
Speaker: (Lecture 1)Susan Gottesman, Ph. D.
Co-chief, Laboratory of Molecular Biology
National Cancer Institute, National Institutes of Health (NCI/NIH)
(Lecture 2) Shinobu Chiba, Ph. D.
Professor, Faculty of Life Sciences and Institute for Protein Dynamics, Kyoto Sangyo University
Title:
(Lecture 1) Post-transcriptional Regulation of the E. coli
    General Stress Response
(Lecture 2) A novel ribosome rescue mechanism in
Bacillus subtilis

Abstract

【Lecture 1】

The general stress response in E. coli allows the bacteria to integrate a large number of stress signals, leading to post-transcriptional up-regulation of the RpoS specialized sigma factor. Induction of RpoS renders cells resistant to many of these stresses, but too much RpoS at the wrong time interferes with cell growth. I will discuss what we know and don’t how about how cells accomplish and coordinate the general stress response, via small RNA-dependent regulation of RpoS translation and anti-adaptors that lead to RpoS stabilization.

( Language: English)

【Lecture 2】

Translation of mRNAs that lack an in-frame stop codon stalls the ribosomes at the 3’ end. Bacteria have evolved several sophisticated mechanisms to rescue such stalled ribosomes. One of them is a release factor (RF)-dependent ribosome rescue factor, which has been reported previously only for a subset of Gram-negative, b- and g-proteobacteria. Here, we report a novel ribosome rescue factor in Bacillus subtilis, a Gram-positive bacterium. We demonstrate that it rescues the stalled ribosome in an RF-dependent manner, implying that it is the first reported example of the RF-dependent ribosome rescue factor in Gram-positive bacteria. Comparison between independently evolved RF-dependent ribosome rescue factors in Gram-positive and Gram-negative bacteria reveals intriguing convergent schemes of the ribosome rescue mechanism and gene regulation.

( Language: English)

Invitator Lab. of Biological membrane system
Yoshinori Akiyama(TEL:075-751-4040)