Date:	November 8, (Fri) 2019 ( Lecture 1)13:30～14:50 (Lecture2)15:00～15:45
Room:	Seminar Room 104 , 1st floor, Bldg. #2 of Institute for Frontier Life and Medical Sciences ,Kyoto University
Speaker:	(Lecture 1)Susan Gottesman, Ph. D. Co-chief, Laboratory of Molecular Biology National Cancer Institute, National Institutes of Health (NCI/NIH) (Lecture 2) Shinobu Chiba, Ph. D. Professor, Faculty of Life Sciences and Institute for Protein Dynamics, Kyoto Sangyo University
Title:	(Lecture 1) Post-transcriptional Regulation of the E. coli 　　　 General Stress Response (Lecture 2) A novel ribosome rescue mechanism in Bacillus subtilis

Abstract

Translation of mRNAs that lack an in-frame stop codon stalls the ribosomes at the 3’ end. Bacteria have evolved several sophisticated mechanisms to rescue such stalled ribosomes. One of them is a release factor (RF)-dependent ribosome rescue factor, which has been reported previously only for a subset of Gram-negative, b- and g-proteobacteria. Here, we report a novel ribosome rescue factor in Bacillus subtilis, a Gram-positive bacterium. We demonstrate that it rescues the stalled ribosome in an RF-dependent manner, implying that it is the first reported example of the RF-dependent ribosome rescue factor in Gram-positive bacteria. Comparison between independently evolved RF-dependent ribosome rescue factors in Gram-positive and Gram-negative bacteria reveals intriguing convergent schemes of the ribosome rescue mechanism and gene regulation.

( Language: English）

Invitator	Lab. of Biological membrane system
	Yoshinori Akiyama（TEL:075-751-4040）