| 2025年3月10日 【1st mechanosensory physiology seminar】Heterogeneous ECM dynamics regulate cell survival and abdominal epithelium remodeling in Drosophila |
| 日時： | 2025年3月10日 11：00～12：00 |
|---|---|
| 場所： | 医生研2号館1階セミナー室（104室） |
| 演者： | Kevin Yuswan, Laboratory for Histogenetic Dynamics, Graduate School of Life Sciences, Tohoku University, Japan |
| 演題： | Heterogeneous ECM dynamics regulate cell survival and abdominal epithelium remodeling in Drosophila |
講演要旨
In Drosophila metamorphosis, the abdominal larval epidermal cells (LECs) rapidly undergo
apoptosis and replaced by adult histoblasts within 20 hours in two distinct phases. During
the first 5 hours, the early phase is characterized by infrequent, isolated LEC apoptosis and
slow histoblast proliferation. This is followed by the late phase, characterized by mass LEC
apoptosis, occasionally in clusters, to spatially accommodate the rapidly proliferating
histoblasts. However, it remains unclear what upstream regulator coordinates the opposing
cell fates of LEC apoptosis and histoblast proliferation. In this study, we demonstrate that the
basal extracellular matrix (ECM) components underlying LECs and histoblasts exhibit distinct
dynamic behaviors. We discovered that the ECM underlying the epithelium rapidly degrades
early during LEC elimination. The loss of ECM beneath LECs also reduces EGFR/ERK
signaling activity, promoting the occurrence of clustered LEC apoptosis in the late phase.
Genetic prevention of ECM degradation delayed LEC elimination by maintaining high EGFR
levels and diminishing clustered apoptosis. In parallel, while other ECM components
underlying histoblasts also continuously degrade, laminins initially degrade partially and then
expand together with the histoblasts. We hypothesize that the new laminin deposition or
expansion act as a scaffold for the rapid histoblast proliferation. These findings suggest that
the spatiotemporally heterogeneous dynamics of ECM components coordinate the precise
molecular mechanisms underlying LEC apoptosis and histoblast expansion to complete the
tissue replacement process in the Drosophila abdominal epithelium.
Reference article: Yuswan, Sun, Kuranaga, Umetsu, PLOS biology, 2024
Host: Keiko Nonomura(nonomura_keiko[@]infront.kyoto-u.ac.jp)