日時：	2025年11月28日（金）　15：00～16：00
場所：	医生物学研究所　医生研１号館1階会議室（134室） Conference Room (Room 134), 1st Floor, Bldg. No.1
演者：	Akira Murakami, Ph.D. Assistant Professor Department of Integrative Physiology School of Pharmaceutical Sciences University of Shizuoka
演題：	Membrane Dynamics-Mediated Regulation of Muscle Satellite Cell Functions

講演要旨

Muscle satellite cells (MuSCs) are muscle-resident stem cells essential for the regeneration of myofibers. During
myofiber injury, the activation and differentiation states of MuSCs are dynamically and precisely controlled. I
refer to this adaptative capacity as the “resilience” of MuSCs and aim to understand its physicochemical basis.
In particular, I hypothesize that the cell membrane—the interface between the cell and its surrounding
microenvironment—acts as a key regulatory factor. Skeletal muscle is characterized by two notable features:
(1) constant exposure to mechanical stress, and (2) being the largest heat-producing organ in the body. By
analyzing the relevant physicochemical parameters at the single-cell level, I attempt to uncover the molecular
mechanisms underlying MuSC resilience.
I have established ex vivo single-cell imaging approaches using fluorescence lifetime microscopy to
quantify membrane tension and intracellular temperature in MuSCs. I found that these parameters change in a
cell-autonomous manner during activation and differentiation. Importantly, these changes coincided with
alterations in membrane lipid composition and in expression of lipid-regulating genes. Furthermore,
perturbating these lipid-related factors impaired the proper progression of MuSC activation and differentiation.
I am currently trying to demonstrate that the regulation of these physicochemical properties directly governs
MuSC fate determination. I would like to discuss how my single-cell biophysical approaches can be integrated
with the current wave of omics frameworks to achieve a synergistic understanding of MuSC resilience.

20251128_Murakami_UoS

Host:
Hirofumi Shintaku, shintaku[@]infront.kyoto-u.ac.jp