日時：	2018年10月10日 14:00~15:00
場所：	京都大学 ウイルス再生研２号館 1階セミナー室
演者：	Maxwell Wilson Ph.D. (Jared Toettcher’s lab. Princeton University)
演題：	Tracing information flow from Erk to target gene induction reveals mechanisms of dynamic and combinatorial control

講演要旨

Cell signaling networks coordinate specific patterns of protein expression in response to external cues. Yet the logic by which signaling pathway activity determines the eventual abundance of target proteins is complex and poorly understood. Here, we describe an approach for simultaneously controlling the Ras/Erk pathway while monitoring a target gene’s transcription and protein accumulation in single live cells. We apply our approach to dissect how Erk activity is decoded by immediate-early genes (IEGs). We find that IEG transcription decodes Erk dynamics through a shared band-pass filtering circuit: repeated Erk pulses transcribe IEGs more efficiently than sustained Erk inputs. However, despite highly similar transcriptional responses, each IEG exhibits dramatically different protein-level accumulation, demonstrating a high degree of post-transcriptional regulation by combinations of multiple pathways. Our results demonstrate that the Ras/Erk pathway is decoded both by dynamic filters and logic gates to shape target gene responses in a context-specific manner.

 

(Mol Cell 2017, 67, 757-769)　　　　　     （言語:英語）

 

 

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