日時：	9:30 - 11:00, May 23 (Tue), 2023
場所：	Room 501, 5th floor, Bldg. #3 Institute for Life and Medical Sciences, Kyoto University
演者：	Dr. Yuhei Goto National Institute for Basic Biology, National Institutes of Natural Sciences, Japan.
演題：	Efficient biosynthesis of linear tetrapyrroles for near-infrared imaging and red optogenetics

講演要旨

Phytochromes are photoreceptors, which have been engineered for their utility as near-infrared fluorescent proteins and optogenetic tools. However, phytochromes requires linear tetrapyrroles, such as phycocyanobilin (PCB), as chromophores, which are not naturally produced in non-photosynthetic organisms like mammalian cells.

To address this limitation, we develop genetically encoded biosynthesis system of PCB (SynPCB), which enables the use of phytochrome-based imaging and optogenetics tools. Heterologous expression of modified four cyanobacterial genes resulted in the efficient synthesis of PCB within mammalian cells and fission yeast cells. Interestingly, near-infrared fluorescent protein (iRFP) fluorescence was greatly enhanced by PCB rather than authentic chromophore. The biosynthesis of PCB also enables the use of phytochrome-based optogenetics in mammalian cells and fission yeast cells upon red light. Using this system, we developed a light-controlled system to reversibly regulate the cell cycle and intracellular signalling. I will talk the history of our SynPCB system development and application for cell biology.　(Language: English)

 

contact: Atsushi Mochizuki

Laboratory of Mathematical Biology,

Institute for Life and Medical Sciences,

Kyoto University

Email: mochi*infront.kyoto-u.ac.jp（*を@へ変えてください）

Tel: 075-751-4612