日時：	October 3rd 1:00-2:00pm
場所：	Conference Room 1st floor, Bldg No.1 of LiMe Zoom: https://kyoto-u-edu.zoom.us/j/82389382827?pwd=WTFXM25ZM1FYU0xiSTRsM010K0tBQT09
演者：	Prof. Dino DI CARLO Bioengineering Department University of California, Los Angeles (UCLA), U.S.A.
演題：	Single-cell Functional Cytometry using Lab on a Particle Technology

講演要旨

We have developed 3D shaped hydrogel microparticle platforms as cel l sized test tubes that
interface with cells and assay cellular function. These “lab on a particle” systems enable s orting
cells based on secreted products for the discove ry of antibodies a nd T cell receptors. Nanovial
particles capture cells and their secretions and are compatible with standard cytometers and
single cell sequencing instruments, linking cell secretory function to transcriptomes for thousands
of single cells . I will discuss o ur latest results linking the transcriptomes of single cells to the
secreted products they produce. I will cover a few example applications of secretion encoded
single cell sequencing (SEC seq) workflow s . In a first example we uncover gene networks
associa ted with high secretion of immunoglobulin G in human plasma cells , one of the most highly
secreting cell types in the body . In a second application we characterize a unique
transcriptionally defined cluster of mesenchymal st em cells ( that secrete higher levels of
vascular endothelial growth factor A (VEGF A and uncover new surface markers that can be used
to select these highly secretory cells . VEGF A is a pro regenerative growth factor that is thought to
drive therapeutic benefit in MSC based therapeutics. I will summarize the potential for nanovial
technology to promote understanding and engineering of functional properties in single cells, and
ultimately drive the next generation of cell therapies.

Host: Lab. of Nano Bioengineering　Hirofumi Shintaku (shintaku*infront.kyoto u.ac.jp) （＊を＠へ変えてください）