2023年5月23日 【Theoretical Biology Seminar】Efficient biosynthesis of linear tetrapyrroles for near-infrared imaging and red optogenetics |
日時: | 9:30 - 11:00, May 23 (Tue), 2023 |
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場所: | Room 501, 5th floor, Bldg. #3 Institute for Life and Medical Sciences, Kyoto University |
演者: | Dr. Yuhei Goto National Institute for Basic Biology, National Institutes of Natural Sciences, Japan. |
演題: | Efficient biosynthesis of linear tetrapyrroles for near-infrared imaging and red optogenetics |
講演要旨
Phytochromes are photoreceptors, which have been engineered for their utility as near-infrared fluorescent proteins and optogenetic tools. However, phytochromes requires linear tetrapyrroles, such as phycocyanobilin (PCB), as chromophores, which are not naturally produced in non-photosynthetic organisms like mammalian cells.
To address this limitation, we develop genetically encoded biosynthesis system of PCB (SynPCB), which enables the use of phytochrome-based imaging and optogenetics tools. Heterologous expression of modified four cyanobacterial genes resulted in the efficient synthesis of PCB within mammalian cells and fission yeast cells. Interestingly, near-infrared fluorescent protein (iRFP) fluorescence was greatly enhanced by PCB rather than authentic chromophore. The biosynthesis of PCB also enables the use of phytochrome-based optogenetics in mammalian cells and fission yeast cells upon red light. Using this system, we developed a light-controlled system to reversibly regulate the cell cycle and intracellular signalling. I will talk the history of our SynPCB system development and application for cell biology. (Language: English)
contact: Atsushi Mochizuki
Laboratory of Mathematical Biology,
Institute for Life and Medical Sciences,
Kyoto University
Email: mochi*infront.kyoto-u.ac.jp(*を@へ変えてください)
Tel: 075-751-4612