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ホーム > SEMINAR / EVENT > 【Medical Chemistry Seminar】Deconvolution of steady state and perturbed hematopoesis by fate mapping and barcoding
2022年12月12日
【Medical Chemistry Seminar】Deconvolution of steady state and perturbed hematopoesis by fate mapping and barcoding
日時: 2022年12月12日(月)Dec 12 (Mon), 2022 17:00~18:30
場所: 医学部A棟103・107室
Seminar Room 103, 107 Building A
The seminar will be held in a hybrid style
演者: Prof. Hans-Reimer Rodewald
Department for Cellular Immunology,
German Cancer Research Center, Heidelberg.
演題: Deconvolution of steady state and perturbed hematopoesis by fate mapping and barcoding

講演要旨

The development of non-invasive fate mapping techniques and high-resolution endogenous DNA
barcoding (Polylox) has enabled insights into the physiology of the blood and immune system. In
addition to hematopoietic stem cell (HSC) differentiation rates (output), major HSC fate groups
have been identified, i.e. multilineage (myelo-erythroid and lymphoid), myelo-erythroid-restricted,
and inactive, i.e. no known progeny, in addition to rare megakaryocyte-restricted HSC. Serial
sampling over the mouse age suggests that these fate patterns are stable over time for individual
HSC clones. RNA-barcoding (PolyloxExpress) can directly link fate measurements with
transcriptome reading in HSC clones and single HSC, which yielded transcriptional signatures
associated with fate patterns. These approaches are now applied to deconvolute embryonic and
adult hematopoiesis, including the development of tissue resident myeloid cells, of innate
lymphocytes, and responses to infection and other hematopoietic stressors. Finally, we are
working towards linkage of HSC fates and fate-associated cellular phenotypes to gain access to
the molecular determinants of fates.

Contact: Prof. Osamu Takeuchi
E-mail: otake*mfour.med.kyoto-u.ac.jp(*を@に変えてください。)
Hosted by Department of Medical Chemistry
Graduate School of Medicine, Kyoto University