2019年11月08日 第1308回 【講演1】Post-transcriptional Regulation of the E. coli General Stress Response / 【講演2】A novel ribosome rescue mechanism in Bacillus subtilis |
日時: | 2019年11月8日(金)【 講演1】13:30~14:50 【 講演2】15:00~15:45 |
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場所: | 京都大学 ウイルス再生研2号館(旧ウイルス研本館) 1階 セミナー室 104 |
演者: | (Lecture 1)Susan Gottesman, Ph. D. Co-chief, Laboratory of Molecular Biology National Cancer Institute, National Institutes of Health (NCI/NIH) (Lecture 2) Shinobu Chiba, Ph. D. Professor, Faculty of Life Sciences and Institute for Protein Dynamics, Kyoto Sangyo University |
演題: | 【講演1】Post-transcriptional Regulation of the E. coli General Stress Response 【講演2】A novel ribosome rescue mechanism in Bacillus subtilis |
講演要旨
【講演 1】
The general stress response in E. coli allows the bacteria to integrate a large number of stress signals, leading to post-transcriptional up-regulation of the RpoS specialized sigma factor. Induction of RpoS renders cells resistant to many of these stresses, but too much RpoS at the wrong time interferes with cell growth. I will discuss what we know and don’t how about how cells accomplish and coordinate the general stress response, via small RNA-dependent regulation of RpoS translation and anti-adaptors that lead to RpoS stabilization.
(言語:英語 Language: English)
【講演 2】
Translation of mRNAs that lack an in-frame stop codon stalls the ribosomes at the 3’ end. Bacteria have evolved several sophisticated mechanisms to rescue such stalled ribosomes. One of them is a release factor (RF)-dependent ribosome rescue factor, which has been reported previously only for a subset of Gram-negative, b- and g-proteobacteria. Here, we report a novel ribosome rescue factor in Bacillus subtilis, a Gram-positive bacterium. We demonstrate that it rescues the stalled ribosome in an RF-dependent manner, implying that it is the first reported example of the RF-dependent ribosome rescue factor in Gram-positive bacteria. Comparison between independently evolved RF-dependent ribosome rescue factors in Gram-positive and Gram-negative bacteria reveals intriguing convergent schemes of the ribosome rescue mechanism and gene regulation.
(言語:英語 Language: English)
主 催 | 京都大学ウイルス・再生医科学研究所 |
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連絡先 | 生体膜システム分野 秋山 芳展(TEL:075-751-4040) |